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    ICSI procedure

    ICSI procedure

    ICSI (Intracytoplasmic Sperm Injection) is performed as a part of IVF. It is conducted when a small number of sperm is available and enables fertilization of an egg with even a single sperm cell.

    ICSI procedure is often recommended if: 

    • the male partner has a very low sperm count
    • other problems with the sperm have been identified, such as poor morphology (abnormally shaped) and/or poor motility (poor swimmers)
    • at previous attempts at in vitro fertilisation (IVF) there was either failure of fertilisation or an unexpectedly low fertilisation rate
    • the male partner has had a vasectomy and sperm have been collected from the testicles or epididymis (sperm reservoir)
    • other situations where the sperm count is zero and donor insemination is not wanted
    • the male partner does not ejaculate any sperm but sperm have been collected from the testicles
    • the male partner has had problems obtaining an erection and ejaculating. This includes men with spinal cord injuries, diabetes and other disorders.

    The procedure for ICSI is similar to that for IVF, but instead of fertilisation taking place in a dish, the embryologist selects sperm from the sample and a single sperm is injected directly into each egg.

    For women

    Step 1. You take fertility drugs to stimulate your ovaries to produce more eggs, as for IVF.

    Step 2. The eggs are then collected and each egg is injected with a single sperm from your partner or a donor. After two to three days in the laboratory, those that are fertilized are transferred to your womb in the same way as for conventional IVF. Any suitable remaining embryos can be frozen for future use.

    Step 3. Clinic may also offer blastocyst transfer, where the fertilized eggs are left to mature for five to six days and then transferred.

    Step 4. After the treatment, clinic will arrange a date with you for your pregnancy test.

    For men

    Step 1. An embryologist will examine your sperm under a microscope and decide whether ICSI could increase your chances of fathering a baby.

    Step 2. The next step depends on whether you are able to provide sperm without medical intervention. If you can, you produce a fresh sperm sample on the same day as your partner’s eggs are collected.

    Step 3. A single sperm is injected into each egg. This does not mean that the egg is fertilized, but ICSI now gives an opportunity for that complex process to commence. ICSI is not a guarantee that fertilization will take place.

    Step 4. Subsequently one – three of the best quality embryos are transferred to the womb.

    In case of zero sperm count

    If you have a zero sperm count (other than caused by vasectomy), the chances of retrieving sperm surgically may be very low or at least uncertain. In this situation, consider having a surgical retrieval such as a ‘dummy run’ and store any sperm that are obtained. If no sperm are retrieved the options of having donor insemination or in vitro fertilization with donor sperm can be considered instead.